Australians stranded in the Chinese city of Wuhan are expected to fly out of the city towards Christmas Island on Monday.
A Qantas flight bound for Wuhan via Hong Kong left Sydney on Sunday afternoon.
The flight is operated by a volunteer team of four pilots and 14 Qantas cabin crew, and will undergo several cautionary procedures both in-flight and once it's landed.
Passengers will go through health checks before boarding and will wear surgical masks.
There will be a limited food and beverage service to minimise interaction between crew and passengers and the 747 plane will be thoroughly cleaned afterwards.
Federal health minister Greg Hunt confirmed an Australian medical assistance team with a mobile hospital had also arrived in the former detention centre of Christmas Island.
"Personnel are in place to receive the passengers from Wuhan and we expect that the flight will be collecting within the next 24 hours," Mr Hunt told reporters in Canberra on Sunday.
The health minister also confirmed that more strict screenings will be in place for passengers arriving not only from Hubei province but all mainland China.
People who have been in mainland China since the start of February, excluding Hong Kong, Macau and Taiwan, are now advised to self-isolate.
Australians are also being told not to travel to mainland China.
Prime Minister Scott Morrison announced on Saturday foreign travellers who have left or passed through China will be denied entry to Australia aiming to stop the spread of the coronavirus.
Australian citizens, permanent residents and their immediate families, dependents, legal guardians and spouses, will be exempted from the strict measures, Mr Morrison said.
"If you've been in mainland China from the 1st of February and you're not an Australian citizen [...] do not travel to Australia at this time," Border Force Commissioner Michael Outram said.
"If you arrive in Australia ... your visa will be cancelled and you will be placed in an alternative place of detention for a quarantine period."
Mr Outram told reporters 71 passengers were not allowed to board their plane in China overnight and 12 flights have been cancelled on Sunday.
As a result of this decision, Football Federation Australia advised the Asian Football Confederation it will now not be possible to play the first match of the AFC Champions League 2020 in Australia.
There have been 12 confirmed cases of coronavirus in Australia
There are now more than 14,000 cases of the virus globally, with just over 300 deaths.
