A Queensland community legal centre will continue to examine the lawfulness of Adani's Carmichael mega mine after the Federal Court dismissed two bids to stop it from going ahead.
Appeals lodged by the Australian Conservation Foundation and traditional land owner Adrian Burragubba were on Friday dismissed by the Federal Court of Australia.
However, Environmental Defenders Office Queensland chief executive Jo Bragg said it could appeal over the ruling in the High Court of Australia.
Ms Bragg could not confirm if that was the final legal option open to opponents of the Indian mining giant's $22 million project but said: "We're still very much examining all aspects of the project and its lawfulness."
Mr Burragubba had appealed against the National Native Title Tribunal's decision to allow the state government to issue a mining lease for the proposed Galilee Basin site.
Meanwhile, the ACF had sought to argue federal Environment Minister Josh Frydenburg did not consider the impact the mine's emissions would have on the Great Barrier Reef under the Environmental Protection and Biodiversity Act.
Adani Australia chief executive Jeyakumar Janakaraj welcomed the rulings.