Pauline Hanson has declared herself a threat to Australia's two-party system after her 10th loss at the ballot box.
Ms Hanson's latest attempt to re-enter politics was shut down on Friday after Queensland electoral commissioner Walter van der Merwe denied her request for a recount in the seat of Lockyer, west of Brisbane.
The 60-year-old narrowly lost to Liberal National incumbent Ian Rickuss by 184 votes, according to the Electoral Commission of Queensland website.
But Ms Hanson said the margin was an even slimmer 114 votes.
She had demanded a recount, citing issues with the way preferences were distributed from the ballots of Labor voters during a notional two-candidate count.
"I think it's an unfair way of counting, and it's not going on the order of preference that the voter wants," Ms Hanson said on Friday.
"It's not just in my seat - I think the whole system needs to overhauled on how they distribute the preferences."
Ms Hanson denied she was casting accusations at the ECQ but called for an investigation, saying present arrangements left the door open to corruption.
She also raised concerns about whether the identities of postal voters were adequately verified.
Ms Hanson has unsuccessfully contested five federal elections and four state polls since 1998. She also lost an election for local government in 1995.
During the 2010 federal election, she signalled an interest in running as a Liberal candidate if she was invited by then opposition leader Tony Abbott.
Mr Abbott made no approach.
The latest defeat appears to have done little to deter her.
"I will be leading the party into the next federal election," she said.
Ms Hanson said support for One Nation remained strong.
Her narrow Lockyer defeat came despite competing against a sitting member of 10 years and being the only candidate to be ignored on Labor's how-to-vote cards, she said.
"The two-party system is what they want to keep in this state and this country and ... they see me as a threat to them."
